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Table 3 Effects of treatment for 24 h with PHA (10 μg/mL) on cell number, viability, and relative gene expression of GSTM2 and CAT (based on GAPDH) in freshly isolated PBMC and cryopreserved PBMC

From: Gene expression profiles in human peripheral blood mononuclear cells as biomarkers for nutritional in vitro and in vivo investigations

 

Relative gene expression

Cell number (106/ml)

Viability (%)

GSTM2

CAT

Mean

SD

Mean

SD

Mean

SD

Mean

SD

Fresh

Medium control

3.97a

1.05

96c

2

1.00e

0.00

1.00g

0.00

PHA

3.56b

0.51

94d

4

0.44e

0.09

0.18g

0.01

Cryopreserved

Medium control

1.83a

0.67

82c

9

1.00f

0.00

1.00h

0.00

PHA

1.26b

0.20

71d

3

0.41f

0.19

0.24h

0.06

  1. Statistical analysis was performed with one-way ANOVA and Bonferroni’s multiple comparison test with selected pairs, and differences between fresh and cryopreserved cells were tested with two-way ANOVA, same letters indicate significant differences, n = 3