Table 6 Putative carbohydrate-modifying enzymes identified in the genome sequences of Bifidobacterium
EC number | Enzyme name | B. adolescentis ATCC15703 | B. adolescentis L2-32 | B. angulatum DSM20098 | B. animalis subps. lactis AD1011 | B. animalis subps. lactis BI-04 | B. animalis subps. lactis HN019 | B. bifidum NCUMB41171 | B. breve DSM20213 | B. catenulatum DSM16992 |
|---|---|---|---|---|---|---|---|---|---|---|
Hexosyltraspherases | ||||||||||
EC:2.4.1.1 | Phosphorylase | 1 | 1 | 1 | 1 | NA | 1 | 1 | – | 1 |
EC:2.4.1.7 | Sucrose phosphorylase. | 1 | – | 1 | 1 | 1 | 1 | – | 1 | 1 |
EC:2.4.1.18 | 1,4-alpha-glucan branching enzyme | 1 | 1 | 1 | 1 | NA | – | 1 | 1 | 1 |
EC:2.4.1.25 | 4-alpha-glucanotransferase | 2 | 2 | 2 | 2 | NA | 2 | 1 | 2 | 2 |
EC:2.4.1.230 | Kojibijose phosphorylase | – | – | – | – | NA | – | – | – | – |
Phosphotrasferases | ||||||||||
EC:2.7.1.12 | Gluconokinase. | 1 | 1 | – | – | NA | – | – | – | 1 |
EC:2.7.1.15 | Ribokinase. | 1 | 1 | 2 | 1 | NA | 1 | – | 2 | 1 |
EC:2.7.1.17 | Xylulokinase. | 1 | 1 | 1 | 1 | NA | 1 | – | – | 1 |
EC:2.7.1.31 | Glycerate kinase. | 1 | 1 | 1 | – | NA | – | 1 | 1 | 1 |
EC:2.7.1.4 | Fructokinase. | 1 | 1 | 1 | 1 | NA | 1 | 1 | 1 | 1 |
EC:2.7.1.6 | Galactokinase. | 1 | 2 | 1 | 1 | NA | 1 | 1 | 6 | 1 |
EC:2.7.6.1 | Ribose-phosphate diphosphokinase. | 2 | 2 | 2 | 2 | NA | 2 | 2 | 2 | 2 |
Glycosyl hydrolases | ||||||||||
EC:3.2.1.1 | α-amylase. | 1 | 1 | – | 1 | – | 1 | – | 1 | – |
EC:3.2.1.10 | Oligo-1,6-glucosidase. | 1 | 2 | 1 | 1 | 1 | 1 | – | 2 | – |
EC:3.2.1.14 | Chitinase | – | – | – | – | – | – | – | – | – |
EC:3.2.1.18 | Exo-alpha-sialidase | – | – | – | – | NA | – | – | 1 | – |
EC:3.2.1.20 | Alpha-glucosidase. | 3 | 3 | 1 | 3 | 1 | 2 | 1 | 2 | 1 |
EC:3.2.1.21 | Beta-glucosidase. | 5 | 4 | 2 | 3 | 2 | 3 | – | 4 | 4 |
EC:3.2.1.22 | Alpha-galactosidase. | 2 | 4 | 2 | 2 | 2 | 2 | 1 | 1 | 3 |
EC:3.2.1.23 | Beta-galactosidase. | 5 | 5 | 4 | 4 | 1 | 4 | 3 | 4 | 3 |
EC:3.2.1.24 | Alpha-mannosidase | 1 |  | – | – | – | – | – | 2 | 1 |
EC:3.2.1.25 | Beta-mannosidase | – | – | – | – | 1 | – | – | – | – |
EC:3.2.1.26 | Beta-fructofuranosidase | 1 | 1 | 1 | 1 | 1 | 1 | – | 1 | 1 |
EC:3.2.1.31 | Beta-glucuronidase | – | – | – | – |  | – | – | – | – |
EC:3.2.1.35 | Hyaluronoglucosaminidase | – | – | – | – | – | – | 1 | – | – |
EC:3.2.1.37 | Xylan-1,4-beta- xylosidase | 2 | 1 | 1 | 1 | 2 | 1 | – | – | 3 |
EC:3.2.1.4 | Cellulase | 1 | 1 | 1 | – | – | – | – | – | 1 |
EC:3.2.1.41 | Pullulunase | 1 | 1 | 1 | – | NA | – | – | 1 | – |
EC:3.2.1.45 | Glucosylceramidase | 1 | 1 | – | 2 | 2 | 2 | – | – | 1 |
EC:3.2.1.51 | Alpha-L-fucosidase | – | – | – | – | NA | – | – | – | – |
EC:3.2.1.52 | Beta-N-acetylhexosaminidase | 1 | 1 | 1 | 1 | NA | 1 | 3 | 1 | 1 |
EC:3.2.1.54 | Cyclomaltodextrinase | – | – | – | – | NA | – | – | 1 | – |
EC:3.2.1.55 | Alpha-N-arabinofuranosidase | 2 | – | 1 | 1 | 1 | 1 | – | – | 3 |
EC:3.2.1.78 | Mannan endo-1,4-beta-mannosidase | – | – | – | 1 | NA | 1 | – | – | – |
EC:3.2.1.86 | 6-phospho-beta-glucosidase | – | – | – | – | NA | – | 1 | – | – |
EC:3.2.1.89 | Arabinogalactan endo-1,4-beta-galactosidase | – | – | – | – | – | – | – | – | – |
EC:3.2.1.93 | Trehalose-6-phosphate hydrolase | 3 | – | – | – | 1 | – | – | – | – |
EC:3.2.1.96 | Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase | – | – | – | – | NA | – | – | – | – |
Isomerases | ||||||||||
EC:5.3.1.1 | Triosephosphate isomerase | 1 | 1 | 1 | 1 | NA | 1 | 1 | 1 | 1 |
EC:5.3.1.4 | L-arabinose isomerase | 1 | 1 | 1 | 1 | NA | 1 | – | – | 1 |
EC:5.3.1.5 | Xylose isomerase | 1 | 1 | 1 | 1 | NA | 1 | – | – | 1 |
EC:5.3.1.6 | Ribose-5-phosphate isomerase | 1 | 1 | 1 | 1 | NA | 1 | 1 | 1 | 1 |
EC:5.3.1.9 | Glucose-6-phosphate isomerase | 1 | 1 | 1 | 1 | NA | 1 | 1 | 1 | 1 |
EC number | Enzyme name | B. dentium ATCC27678 | B. gallicum DSM20093 | B. longum subps. longum ATCC15697 | B. longum subsp. Infantis ATCC55813 | B. longum subsp. Infantis CCUG52486 | B. longum subsp. longum DJO10A | B. longum subsp. longum NCC2705 | B. pseudocatenulatum DSM20438 | |
|---|---|---|---|---|---|---|---|---|---|---|
Hexosyltraspherases | ||||||||||
EC:2.4.1.1 | Phosphorylase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:2.4.1.7 | Sucrose phosphorylase. | 1 | – | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:2.4.1.18 | 1,4-alpha-glucan branching enzyme | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:2.4.1.25 | 4-alpha-glucanotransferase | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
EC:2.4.1.230 | Kojibijose phosphorylase | – | – | – | – | – | – | – | – | |
Phosphotrasferases | ||||||||||
EC:2.7.1.12 | Gluconokinase. | 1 | – | – | 1 | 1 | 1 | 1 | – | |
EC:2.7.1.15 | Ribokinase. | 1 | 1 | 2 | 4 | 2 | 2 | 2 | 1 | |
EC:2.7.1.17 | Xylulokinase. | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | |
EC:2.7.1.31 | Glycerate kinase. | 1 | – | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:2.7.1.4 | Fructokinase. | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | |
EC:2.7.1.6 | Galactokinase. | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:2.7.6.1 | Ribose-phosphate diphosphokinase. | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
Glycosyl hydrolases | ||||||||||
EC:3.2.1.1 | α-amylase. | – | – | 1 | – | – | – | – | – | |
EC:3.2.1.10 | Oligo-1,6-glucosidase. | 1 | 1 | 1 | – | 2 | 1 | 1 | 2 | |
EC:3.2.1.14 | Chitinase | – | – | – | – | – | – | 1 | 1 | |
EC:3.2.1.18 | Exo-alpha-sialidase | – | – | 2 | – | – | – | – | – | |
EC:3.2.1.20 | Alpha-glucosidase. | 4 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | |
EC:3.2.1.21 | Beta-glucosidase. | 12 | 1 | 2 | 3 | 2 | 2 | 1 | 4 | |
EC:3.2.1.22 | Alpha-galactosidase. | 5 | – | 1 | 1 | 1 | 1 | 1 | 2 | |
EC:3.2.1.23 | Beta-galactosidase. | 4 | 1 | 4 | 4 | 3 | 3 | 3 | 5 | |
EC:3.2.1.24 | Alpha-mannosidase | 1 | – | 2 | 2 | – | 3 | 3 | – | |
EC:3.2.1.25 | Beta-mannosidase | – | – | – | – | – | – | – | – | |
EC:3.2.1.26 | Beta-fructofuranosidase | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:3.2.1.31 | Beta-glucuronidase | 1 | – | – | – | 1 | 1 | – | – | |
EC:3.2.1.35 | Hyaluronoglucosaminidase | – | – | – | – | – | – | – | – | |
EC:3.2.1.37 | Xylan-1,4-beta- xylosidase | 2 | 1 | – | – | 1 | 1 | – | 4 | |
EC:3.2.1.4 | Cellulase | – | – | – | – | – | – | – | 2 | |
EC:3.2.1.41 | Pullulunase | 1 | 1 | – | – | – | – | – | 1 | |
EC:3.2.1.45 | Glucosylceramidase | 1 | – | – | – | – | – | – | 1 | |
EC:3.2.1.51 | Alpha-L-fucosidase | 1 | – | 3 | – | – | – | – | – | |
EC:3.2.1.52 | Beta-N-acetylhexosaminidase | – | 1 | 4 | 2 | 2 | 2 | 2 | 2 | |
EC:3.2.1.54 | Cyclomaltodextrinase | – | – | 1 | 1 | 1 | 1 | 1 | – | |
EC:3.2.1.55 | Alpha-N-arabinofuranosidase | 3 | – | – | 3 | 2 | 3 | 3 | 3 | |
EC:3.2.1.78 | Mannan endo-1,4-beta-mannosidase | 1 | – | – | – | – | – | – | – | |
EC:3.2.1.86 | 6-phospho-beta-glucosidase | – | – | – | – | – | – | – | – | |
EC:3.2.1.89 | Arabinogalactan endo-1,4-beta-galactosidase | – | – | – | – | – | 1 | 1 | – | |
EC:3.2.1.93 | Trehalose-6-phosphate hydrolase | – | – | – | – | – | – | 1 | – | |
EC:3.2.1.96 | Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase | – | – | – | – | – | 1 | 1 | – | |
Isomerases | ||||||||||
EC:5.3.1.1 | Triosephosphate isomerase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:5.3.1.4 | L-arabinose isomerase | 1 | 1 | – | 1 | 1 | 1 | 1 | 1 | |
EC:5.3.1.5 | Xylose isomerase | 1 | 1 | – | 1 | 1 | 1 | 1 | 1 | |
EC:5.3.1.6 | Ribose-5-phosphate isomerase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
EC:5.3.1.9 | Glucose-6-phosphate isomerase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |