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Fig. 5 | Genes & Nutrition

Fig. 5

From: Growth factor TGF-β induces intestinal epithelial cell (IEC-6) differentiation: miR-146b as a regulatory component in the negative feedback loop

Fig. 5

Smad7 is a downstream effector of SIAH2. pCMV-SPORT6-SIAH2 vector was transfected into IEC-6 cells prior to TGF-β treatment. After 36 h transfection, total Smad7 protein was analyzed by immunoblotting. Over-expression of SIAH2 was confirmed by immunoblotting. Values are means ± SEM run in triplicates; letters indicate significant differences between treatment groups (P < 0.05)

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