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Table 3 Gene expression analysis in white adipose tissue (eWAT) of animals in Experiment 1

From: Identification of Mest/Peg1 gene expression as a predictive biomarker of adipose tissue expansion sensitive to dietary anti-obesity interventions

Gene name

Gene symbol

LFD

HFD

HFD/EGCG

HFD/PUFA

HFD/HP

ANOVA

Lipid metabolism

 ATP citrate lyase

Acly

1.00 ± 0.23a

0.55 ± 0.03a

0.42 ± 0.07b

0.69 ± 0.0.11a

0.23 ± 0.23b

0.002

 ELOVL family member 6

Elovl6

1.00 ± 0.18a

0.48 ± 0.10b

0.33 ± 0.06b

0.45 ± 0.08b

0.17 ± 0.03b

<0.0001

 Fatty acid synthase

Fasn

1.00 ± 0.18

0.91 ± 0.11

0.71 ± 0.09

0.79 ± 0.11

0.50 ± 0.12

ns

 Acetyl-coenzyme A carboxylase alpha

Acaca

1.00 ± 0.13a

0.44 ± 0.02b

0.52 ± 0.07b

0.62 ± 0.08b

0.40 ± 0.05b

<0.0001

 Malic enzyme 1

Me1

1.00 ± 0.21a

0.71 ± 0.10ab

0.49 ± 0.06ab

0.79 ± 0.13ab

0.38 ± 0.06b

0.01

 Patatin-like phospholipase domain-containing protein 2 (adipose triglyceride lipase)

Pnpla2

1.00 ± 0.07ab

1.14 ± 0.13a

0.60 ± 0.05b

1.04 ± 0.10a

0.88 ± 0.12ab

0.004

Carbohydrate metabolism

 Glycogen synthase 2

Gys2

1.00 ± −0.17ab

1.19 ± 0.26ab

0.70 ± 0.10ab

1.67 ± 0.37a

0.66 ± 0.17b

0.02

 Solute carrier family 2 (facilitated glucose transporter), member 4

Slc2a4

1.00 ± 0.07a

1.64 ± 0.13ab

1.54 ± 0.11ab

0.92 ± 0.08a

2.16 ± 0.37b

0.0002

 Transketolase

Tkt

1.00 ± 0.12a

0.58 ± 0.06b

0.55 ± 0.06b

0.73 ± 0.10ab

0.48 ± 0.06b

0.0008

Adipokines

 Serine (or cysteine) peptidase inhibitor, clade E, member 1

Serpine1

1.00 ± 0.22

1.36 ± 0.25

0.82 ± 0.14

0.79 ± 0.14

0.69 ± 0.14

ns

 Haptoglobin

Hp

1.00 ± 0.13a

1.22 ± 0.16ab

1.23 ± 0.08ab

1.03 ± 0.08a

1.66 ± 0.21b

0.02

 Retinol-binding protein 4, plasma

Rbp4

1.00 ± 0.14ab

1.45 ± 0.26a

0.49 ± 0.05b

1.42 ± 0.17a

0.55 ± 0.05b

<0.0001

Oxidative phosphorylation

 NADH dehydrogenase (ubiquinone) Fe-S protein 1

Ndufs1

1.00 ± 0.06

0.97 ± 0.05

0.92 ± 0.05

1.04 ± 0.08

0.95 ± 0.05

ns

 ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1

Atp5c1

1.00 ± 0.05

1.17 ± 0.06

0.95 ± 0.04

0.99 ± 0.08

0.99 ± 0.06

ns

Miscellaneous

 Epithelial membrane protein 1

Emp1

1.00 ± 0.06

1.09 ± 0.14

1.37 ± 0.18

0.92 ± 0.14

1.17 ± 0.16

ns

 Transformation-related protein 53-inducible nuclear protein 2

Trp53inp2

1.00 ± 0.07

1.57 ± 0.13

1.41 ± 0.15

1.27 ± 0.15

1.25 ± 0.14

ns

 Thyroid hormone responsive SPOT14 homolog (rattus)

Thrsp

1.00 ± 0.12

1.00 ± 0.10

0.86 ± 0.13

1.36 ± 0.20

1.32 ± 0.17

ns

 Protein phosphatase 1, regulatory (inhibitor) subunit 1A

Ppp1r1a

1.00 ± 0.07

0.98 ± 0.11

0.93 ± 0.07

1.32 ± 0.16

0.94 ± 0.11

ns

 Cell death-inducing DNA fragmentation factor, alpha subunit-like effector A

Cidea

1.00 ± 0.13a

0.52 ± 0.09b

0.59 ± 0.06b

0.64 ± 0.09ab

0.45 ± 0.05b

0.001

 Insulin-induced gene 1

Insig1

1.00 ± 0.15a

1.02 ± 0.10a

0.67 ± 0.10ab

1.00 ± 0.15a

0.42 ± 0.08b

0.003

  1. Data are mean ± SEM (n = 7–8). Significant differences between groups were tested by one-way ANOVA and least-significant difference (LSD) post hoc comparisons (P < 0.05). Values not sharing a common letter are statistically different