Fig. 1

Expression levels of four genes involved in the NF-κB pathway. Confluent HT-29 cells were cultured for 5 h in media alone (white), TNF-α in media (black) or TNF-α in media with PF (grey) using three replicate samples for each treatment. Following culture RNA was extracted, and four of the dysregulated genes were selected and were quantified by RT-PCR. RT-PCR gene expression was normalised to housekeeping gene β₂-microglobulin. In good accordance with the microarray data, all genes became significantly overexpressed following TNF-α treatment (black) [vs. untreated cells (white); *P < 0.05 for all comparisons]. In further accordance with the microarray data, only expression of TNF was significantly downregulated by PF treatment (grey) (vs. TNF-α treated; ^# P < 0.05). PF polymeric formula: genes—ICAM1 intercellular adhesion molecule 1, IL-8 interleukin-8, NFKBIE NF-κB light polypeptide gene enhancer in B cells inhibitor, α, TNF tumour necrosis factor. Data presented as mean with SD