Fig. 2

IL-8 and IL-6 protein levels in cell culture supernatants. Confluent HT-29 cells were incubated with media alone or media with 100 ng/ml of TNF-α in the presence or absence of PF for 5 h with supernatants collected following incubation. Experiments were performed using three replicate samples for each treatment on two independent occasions. IL-8 (a) and IL-6 (b) protein levels were measured in the supernatants by ELISA. After TNF-α exposure, IL-8 and IL-6 levels were significantly elevated (vs. untreated cells; *P < 0.05). However, PF had anti-inflammatory effects on HT-29 in response to TNF-α. The PF-treated cells had IL-8 and IL-6 levels below the TNF-α-treated cells (^# P < 0.05). Data presented as mean with SD