Fig. 2

MAD2L1 mRNA and protein expression. a Alignment between miR-192-5p and predicted target MAD2L1 using miRTarBase software. b Expression level of MAD2L1 mRNA in duodenal biopsies of controls (n = 8), Marsh 3AB (n = 8) and Marsh 3C (n = 12) celiac pediatric patients. Data obtained by quantitative PCR are represented as fold chance compared to an external sample. The box plots show median value and the 25 and 75 percentiles, whiskers the maximum and minimum value. Statistical analysis was performed by ANOVA. c Densitometric analysis of MAD2L1 protein in duodenal biopsies of celiac patients and controls normalized on actin expression (n = 7 controls, n = 8 Marsh 3AB and n = 8 Marsh 3C) and d western blot image of MAD2L1 and actin levels in controls and CD patients