Fig. 3

FOXP3 mRNA and protein, and RUNX1 mRNA expression. a Expression level of FOXP3 mRNA in duodenal biopsies of controls (n = 8), Marsh 3AB (n = 8) and Marsh 3C (n = 12) celiac pediatric patients. Data obtained by quantitative PCR are represented as fold change compared to an external sample. The box plots show median value and the 25 and 75 percentiles, whiskers the maximum and minimum value. Statistical analysis was performed by ANOVA. b western blot image of FOXP3 and actin levels in controls and CD patients and c densitometric analysis of FOXP3 protein in duodenal biopsies of celiac patients and controls normalized on actin expression (n = 7 controls, n = 7 Marsh 3AB and n = 8 Marsh 3C). d Expression level of RUNX1 mRNA in duodenal biopsies of controls (n = 8), Marsh 3AB (n = 8) and Marsh 3C (n = 12) celiac pediatric patients. Data obtained by quantitative PCR are represented as fold chance compared to an external sample and statistical analysis performed by ANOVA