Fig. 3
The effect of minerals on iron-regulated gene mRNA expression levels in Caco-2 cells under the DFO treatment condition. Caco-2 cells were grown on the 12-well tissue culture plates for 21 days. The cells were treated by 200 μM for 24h to induce iron deficiency. The copper mineral (CuCl2; 100 μM) and iron (FAC; 100 μg/mL) were given to Caco-2 cells for 18h to test the effect of Cu and Cu with Fe on iron- (a) and hypoxia- (b)regulated genes mRNA expressions