Dietary supplementation with methyl donors reduces fatty liver and modifies the fatty acid synthase DNA methylation profile in rats fed an obesogenic diet

Table 2 Weight-related and plasma measurements of female Wistar rats after 8 weeks of dietary treatments

	Female dietary groups
	C (n = 7)	HFS (n = 9)	HFSsupp (n = 10)	ANOVA
Weight-related measurements
Weight gain (g)	68.7 ± 5.9a	102.8 ± 8.9b	109.4 ± 11.4b	*
Subcutaneous WAT ( %)	1.40 ± 0.14a	2.02 ± 0.26a,b	2.27 ± 0.28b	*
Retroperitoneal WAT ( %)	1.87 ± 0.19a	2.70 ± 0.29b	3.09 ± 0.24b	**
Epididymal WAT (%)	2.42 ± 0.30a	3.53 ± 0.49a	3.84 ± 0.41a	ns
Mesenteric WAT (%)	1.28 ± 0.18a	1.63 ± 0.19a	1.62 ± 0.21a	ns
Liver (%)	2.18 ± 0.03a	2.14 ± 0.06a	2.24 ± 0.07a	ns
Gastrocnemius muscle (%)	0.59 ± 0.02a	0.55 ± 0.03a	0.51 ± 0.04a	ns
Plasma values
Glucose (mg/dL)	103.4 ± 6.1a	108.9 ± 3.7a	119.3 ± 6.7a	ns
HDL cholesterol (mg/dL)	56.8 ± 4.3a	40.4 ± 4.6b	54.2 ± 4.5a	*
Triglycerides (mg/dL)	86.5 ± 6.9a	57.7 ± 5.3a	74.1 ± 13.2a	ns
Lactate (mg/dL)	27.8 ± 4.3a	26.1 ± 3.4a	34.3 ± 4.7a	ns
Homocysteine (μmol/L)	15.6 ± 1.1a	15.2 ± 0.8a	14.1 ± 0.9a	ns
Insulin (μU/mL)	15.5 ± 4.7a	16.4 ± 3.3a	21.2 ± 4.5a	ns

All the results are expressed as the mean ± SE. Statistical analyses were performed using one-way Anova and Tukey post hoc test. Different letters (a, b) indicate significant differences between groups of at least p < 0.05
WAT white adipose tissue, C control group, HFS high-fat-sucrose group, HFSsupp high-fat-sucrose supplemented with methyl donors group, ns non statistically significant
* p < 0.05, ** p < 0.01

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