Fig. 2

Lipid analysis and differential expression of lipogenic genes in adult wild-type worms and sams-1 mutants. a TLC-based analysis of PC and PE in wild-type (WT) worms and the sams-1(t3210) and sams-1(ok3033) mutants. The levels of PC and PE were adjusted to the respective body volumes of the worms. b Body volumes of WT and sams-1 mutant worms. The data were derived from analysis of bright field microscopy images. Images of 10 worms per strain were analyzed in 3 independent experiments. c Number of progeny of WT and sams-1 mutant worms. The number of viable L4 progeny per worm was counted over the entire reproductive phase. Five worms per strain were analyzed in three independent experiments. d TLC-based analysis of TAG in WT and sams-1 mutant worms. TAG levels were adjusted to the respective body volumes of the worms. e The expression of selected lipogenic genes was compared between WT and sams-1 mutant worms. Genes up-regulated in the sams-1 mutants are shown in red; down-regulated genes are shown in green. Fold changes in expression intensities were calculated from the average gene expression value of each gene and represent the mutant-to-wild-type ratio. Microarray-based gene expression data were derived from 3 independent experiments. a–d Significant differences were analyzed between WT worms and the respective sams-1 mutant using unpaired t tests (*p < 0.05; **p < 0.01; ***p < 0.001)