Fig. 3

Lipid droplets in wild-type (WT) and sams-1 mutant worms visualized via vital BODIPY™ 493/503 fat staining. a–c DIC images (a1, b1, c1) and fluorescence images (a2, b2, c2) of the anterior intestines of adult WT worms (a1, a2) and adult sams-1(t3210) (b1, b2) and sams-1(ok3033) mutants (c1, c2) are shown. Detailed images of lipid droplets in WT worms (a3) and the sams-1(t3210) (b3) and sams-1(ok3033) (c3) mutants are shown as merged DIC and fluorescence images. d ATGL-1::GFP as a marker of lipid droplets in sams-1(t5669) mutants. Localization of ATGL-1::GFP on the surface of lipid droplets in sams-1(t5669) mutants expressing ATGL-1::GFP. Lipid droplets were stained with LipidToxRed. e, f DIC images (e1, f1) and fluorescence images (e2, f2) of 12-cell embryos of WT (e1, e2) and sams-1(t5669) mutants (f1, f2). Detailed images of lipid droplets in WT worms (e3) and sams-1(t3210) mutants (f3) are shown as merged DIC and fluorescence images. The scale bar represents 50 μm in images a1/2, b1/2, and c1/2; 10 μm in images a3, b3, and c3; 2 μm in image d; 20 μm in e1/2 and f1/2; and 2 μm in images e3 and f3