Fig. 4

Analysis of lipid droplets in the anterior and posterior intestine of adult wild-type (WT) and sams-1 mutant worms. a–f Mean volume (a, b), number (c, d), and total volume (e, f) of LDs in the anterior (a, c, e) and the posterior (b, d, f) intestine of WT worms and the sams-1(t3210) and sams-1(ok3033) mutants. G, H) Distribution of the total LD volume of LD size classes (<2, 2–5, 5–10, >10 μm³) in the anterior (g) and posterior (h) intestine of WT worms and sams-1 mutants. LDs were visualized via BODIPY™ 493/503 fat staining. Images were analyzed using the 3D object counter plugin of ImageJ software. a–h Data were derived from at least 10 individual worms from 3 independent experiments. Statistically significant differences were calculated between WT worms and the respective RNAi-treated mutant strains using unpaired t tests (**p < 0.01; ***p < 0.001)