Fig. 5

Depletion of triacylglyceride and lipid droplet breakdown in response to starvation in wild-type (WT) worms and sams-1 mutants. a Enzymatically determined TAG levels in L4 larvae of WT worms and sams-1(t3210) and sams-1(ok3033) mutants exposed to starvation for 24 or 48 h. b, c The total lipid droplet volume in the anterior (b) and posterior (c) intestine of L4 larvae (0 h) of WT worms and the sams-1(t3210) and sams-1(ok3033) mutants exposed to starvation for 24 or 48 h. d–g Number of LDs classified by size (<2, 2–5, 5–10, 10–30 μm³) in L4 larvae (0 h) of WT worms and the sams-1(t3210) and sams-1(ok3033) mutants in response to starvation (24 or 48 h). The analyzed LDs were localized to the anterior and posterior intestine. b–g LDs were visualized via BODIPY™ 493/503 fat staining, and images were analyzed using the 3D object counter plugin of ImageJ software. Asterisks indicate significant differences between WT worms and the respective sams-1 mutant at a given time point, determined using unpaired t tests with Welch’s correction (*p < 0.05; **p < 0.01; ***p < 0.001)