Table 1 Primer sequences and annealing temperature of primers used for quantitative RT-PCR
Genes | Forward primer 5′–3′ | Reverse primer 5′–3′ | T (°C) |
|---|---|---|---|
Canx* | GCAGCGACCTATGATTGACAACC | GCTCCAAACCAATAGCACTGAAAGG | 58.5 |
Cpt1a | AAAGATCAATCGGACCCTAGACA | CAGCGAGTAGCGCATAGTCA | 60.0 |
Cpt1b | ACCCCTAAGGATGCCATTCTTG | GCGGAAGCACACCAGGCAGTA | 57.0 |
Esrra | ATCCAGGGGAGCATCGAGTA | AAAGGCAAAGGGTCCACCTC | 60.0 |
Fasn | AGTTAGAGCAGGACAAGCCCAAG | TTCAGTGAGGCGTAGTAGACAGTG | 60.0 |
Mpc1 | GACTTTCGCCCTCTGTTGCT | GCAGATGGCCGCTTACTCAT | 58.5 |
Mpc2 | ACCTACCACCGACTCATGGA | AGTTTCTCTGCAGGTCTGGC | 60.0 |
Lipe | TGGAACTAAGTGGACGCAAGCC | TCAAGGTATCTGTGCCCAGTAAGC | 58.0 |
Pdha1 | CTGCCTATTGCAGGTCTGGT | CTTCTCGAGTGCGGTAGCTT | 60.0 |
Pdhb | GAAAGGCAAGGGACCCACAT | CCTCCTTCCACAGTCACGAG | 60.0 |
Pdk1 | GCTACTCAACCAGCACTCCTT | GGTCGCTCTCATGGCATTCT | 60.0 |
Pnpla2 | ACCACCCTTTCCAACATGCTACC | GCTACCCGTCTGCTCTTTCATCC | 58.0 |
Ppargc1a | CCCTGCCATTGTTAAGACC | TGCTGCTGTTCCTGTTTTC | 60.0 |
Rps15* | CGGAGATGGTGGGTAGCATGG | ACGGGTTTGTAGGTGATGGAGAAC | 58.5 |
S100a8 | ACTTCGAGGAGTTCCTTGCG | TGCTACTCCTTGTGGCTGTC | 58.0 |