Table 1 Real-time RT-qPCR primer and probe sequences, amplicon lengths and mRNA variants amplified
From: Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells
Gene | Â |
 POLR2A |  |
  Forward primer | 5′ GCAAATTCACCAAGAGAGACG 3′ |
  Reverse primer | 5′ CACGTCGACAGGAACATCAG 3′ |
  Probe | 5′ [FAM]CCTGGAGC[TAMRA] 3′ |
  Amplicon length | 73 nucleotides |
  mRNA variants amplified | NM_000937.4 |
 DMT-1 (SLC11A2) |  |
  Forward primer | 5′ GATGTAGAGCATCTAACAGGGATGAA 3′ |
  Reverse primer | 5′ CCATTGGCAAAGTCACTCATTACT 3′ |
  Probe | 5′ [FAM]TCTACAGAGCTTACAGCTTCCCTTTGCTCT[TAMRA] 3′ |
  Amplicon length | 128 nucleotides |
  mRNA variants amplified | NM_001174125.1, NM_001174126.1, NM_001174127.1, NM_000617.2, NM_001174128.1, NM_001174129.1, NM_001174130.1 |
 FPN (SLC40A1) |  |
  Forward primer | 5′ GCCCATAATCTCTGTCAGTCTGC 3′ |
  Reverse primer | 5′ TGCCTCTTTCAGATTCAATTACATTT 3′ |
  Probe | 5′ [FAM]AATCAAAGGACCAAAGACCGATTCTAGCA[TAMRA] 3′ |
  Amplicon length | 122 nucleotides |
  mRNA variants amplified | XM_005246504.1, XM_005246505.1 |
 TfR-1 (TFRC) |  |
  Forward primer | 5′ GCTTTCCCTTTCCTTGCATATTCT 3′ |
  Reverse primer | 5′ GGTGGTACCCAAATAAGGATAATCTG 3′ |
  Probe | 5′ [FAM]ATCCCAGCAGTTTCTTTCTGTTTTTGCGA[TAMRA] 3′ |
  Amplicon length | 87 nucleotides |
  mRNA variants amplified | NM_001128148.1, NM_003234.2 |