Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells

Genes & Nutrition

Table 2 Genes differentially expressed in response to addition of holo-Tf to the basolateral medium of confluent Caco-2 cell monolayers from day 14 to day 21 after cell seeding

Affymetrix Probeset ID	Gene	Gene symbol	Fold change^a	Uncorrected p value^b	FDR^c
226837_at	Sprouty-related, EVH1 domain containing 1	SPRED1	2.19	9.77E−06	0.016
208891_at	Dual-specificity phosphatase 6	DUSP6	1.51	1.01E−05	0.016
232321_at	Mucin 17	MUC17	3.61	6.50E−06	0.016
227246_at	Pleiotropic regulator 1	PLRG1	−1.79	1.05E−05	0.016
209101_at	Connective tissue growth factor	CTGF	1.64	6.89E−06	0.016
208383_s_at	Phosphoenolpyruvate carboxykinase 1	PCK1	2.00	4.32E−06	0.016
211357_s_at	Aldolase B	ALDOB	1.48	2.10E−05	0.027

^aPositive values indicate increased expression following holo-Tf treatment (mean expression in holo-Tf-treated cells/mean expression in controls). Negative values indicate decreased expression following holo-Tf treatment (−mean expression in control cells/mean expression in holo-Tf treated cells)
^bDerived from unpaired t tests with Bayesian correction (Limma) comparing data from microarrays for holo-Tf treated and control cells (n = 5 for both treatments)
^cFalse discovery rate

ISSN: 1865-3499