Identification of Mest/Peg1 gene expression as a predictive biomarker of adipose tissue expansion sensitive to dietary anti-obesity interventions

Table 2 Biometric data and plasma analyses in animals of Experiment 1

	LFD	HFD	HFD/EGCG	HFD/PUFA	HFD/HP	ANOVA
Initial body weight (g)	25.4 ± 0.47	25.3 ± 0.59	25.2 ± 0.41	25.3 ± 0.41	25.7 ± 0.46	ns
Final body weight (g)	26.6 ± 0.44	27.6 ± 0.57	26.9 ± 0.53	27.61 ± 0.30	26.49 ± 0.42	ns
Final fat mass (g)	6.74 ± −0.21^a	7.85 ± 0.29^c	7.26 ± 0.24^abc	7.49 ± 0.21^bc	6.85 ± 0.31^ab	0.03
Cumulative energy intake (kJ/animal)	356 ± 7.1^a	419 ± 13^b	362 ± 14^a	400 ± 7.4^ab	423 ± 16^b	0.0002
eWAT weight (% BW)	1.81 ± 0.07	2.04 ± 0.15	1.70 ± 0.08	1.85 ± 0.14	1.67 ± 0.16	ns
Liver weight (% BW)	4.04 ± 0.10	3.93 ± 0.10	3.87 ± 0.06	4.05 ± 0.11	4.33 ± 0.10	ns
Liver TG (mg/mg protein)	0.999 ± 0.164	1.28 ± 0.281	0.934 ± 0.203	0.932 ± 0.162	0.824 ± 0.167	ns
Blood glucose (mmol/L)	6.36 ± 0.21	7.23 ± 0.40	6.80 ± 0.59	7.16 ± 0.41	6.51 ± 0.30	ns
Plasma insulin (µg/L)	1.01 ± 0.14	1.33 ± 0.17	1.66 ± 0.37	1.47 ± 0.25	0.97 ± 0.09	ns
Plasma TG (mmol/L)	0.669 ± 0.121^a	0.364 ± 0.044^b	0.330 ± 0.048^b	0.349 ± 0.046^b	0.436 ± 0.040^ab	0.004
Plasma NEFA (mmol/L)	0.897 ± 0.045	0.840 ± 0.063	0.756 ± 0.084	0.761 ± 0.052	0.905 ± 0.065	ns
Plasma cholesterol (mg/dL)	140 ± 4.5^ab	137 ± 11.1^ab	146 ± 6.7^b	113 ± 10.4^c	119 ± 5.4^ac	0.04

Data are mean ± SEM (n = 8–10). Significant differences between groups were tested by one-way ANOVA and least-significant difference (LSD) post hoc comparisons (P < 0.05). Values not sharing a common letter are statistically different
TG triglycerides, NEFA non-esterified fatty acids

ISSN: 1865-3499