Fig. 7

Immunocytochemical localization of SLC30A5 and SLC30A6. Immunostaining to detect SLC30A5 (a–d) and SLC30A6 (e–h) was carried out on control fibroblasts (a, e) Mother 2 fibroblasts (b, f) PMC-42 (c, g) and cryosections of human breast tissue (d, h). Fibroblasts grown on coverslips and lymphoblasts grown in suspension were fixed with 4 % paraformaldehyde and stained with SLC30A5 and SLC30A6 antibodies, respectively, followed by secondary antibody conjugated with Alexa 488. SLC30A5 and SLC30A6. Images show vesicular cytoplasmic and perinuclear localization of SLC30A5 (a, b, c, d) and SLC30A6 (e, f, g, h) in control fibroblast, Mother 2 fibroblasts, PMC-42LA and human breast tissue, respectively. Bar 20 μm