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Table 1 Influence of mucosal incubation with GABA or glutamine on mRNA expression in porcine jejunum

From: GABA selectively increases mucin-1 expression in isolated pig jejunum

 

MUC1

MUC2

MUC3

MUC4

MUC12

MUC13

MUC20

Control

1.00 ± 0.07

1.00 ± 0.11

1.00 ± 0.16

1.00 ± 0.24

1.00 ± 0.23

1.00 ± 0.24

1.00 ± 0.16

50 µM GABA

2.33 ± 0.40

1.11 ± 0.15

0.96 ± 0.10

1.20 ± 0.21

0.56 ± 0.10

0.84 ± 0.21

0.88 ± 0.17

1 mM GABA

2.04 ± 0.50

1.29 ± 0.09

0.92 ± 0.11

0.90 ± 0.20

0.86 ± 0.11

0.95 ± 0.20

0.83 ± 0.12

P value

0.02

0.14

0.91

0.55

0.12

0.82

0.70

Control

1.00 ± 0.07

1.00 ± 0.11

1.00 ± 0.16

1.00 ± 0.24

1.00 ± 0.23

1.00 ± 0.24

1.00 ± 0.16

1 mM Gln

1.99 ± 0.36

1.07 ± 0.06

0.98 ± 0.12

1.00 ± 0.21

1.03 ± 0.26

0.96 ± 0.18

0.78 ± 0.08

10 mM Gln

1.47 ± 0.26

0.93 ± 0.10

1.27 ± 0.25

0.71 ± 0.15

0.94 ± 0.24

0.71 ± 0.15

0.82 ± 0.16

P value

0.14

0.55

0.68

0.35

0.96

0.52

0.49

 

GLS

GAD65

ABAT

GBR1

ADAM17

MMP14

GPx2

IL10

Control

1.00 ± 0.24

1.00 ± 0.09

1.00 ± 0.29

1.00 ± 0.11

1.00 ± 0.18

1.00 ± 0.17

1.00 ± 0.19

1.00 ± 0.11

50 µM GABA

0.85 ± 0.21

1.48 ± 0.30

0.69 ± 0.15

1.00 ± 0.07

0.86 ± 0.24

1.00 ± 0.19

1.00 ± 0.14

1.17 ± 0.12

1 mM GABA

0.71 ± 0.11

1.14 ± 0.21

0.59 ± 0.11

1.02 ± 0.08

0.78 ± 0.15

0.84 ± 0.21

0.87 ± 0.16

1.08 ± 0.15

P value

0.65

0.46

0.60

0.62

0.71

0.65

0.77

0.47

Control

1.00 ± 0.24

1.00 ± 0.09

1.00 ± 0.29

1.00 ± 0.11

1.00 ± 0.18

1.00 ± 0.17

1.00 ± 0.19

1.00 ± 0.11

1 mM Gln

0.70 ± 0.08

1.57 ± 0.36

0.59 ± 0.08

1.01 ± 0.08

0.68 ± 0.11

0.88 ± 0.14

1.03 ± 0.20

1.13 ± 0.16

10 mM Gln

1.01 ± 0.34

1.21 ± 0.29

0.85 ± 0.22

0.83 ± 0.07

0.92 ± 0.15

1.15 ± 0.18

0.93 ± 0.18

1.13 ± 0.12

P value

0.77

0.80

0.49

0.25

0.30

0.54

0.95

0.68

  1. Data were normalized to beta-2 microglobulin and beta-actin (MUC1, MUC2, GAD65) or GAPDH (other genes) and scaled to the mean of the control group. Values represent mean ± SEM. For abbreviations, see text section “Gene expression analysis”; n = 9
  2. Significant results are highlighted in bold