Fig. 4

Effect of 1,25D on TPH1, TPH2 and Cyp24a1 mRNAs in a serotonergic neuronal cell line. P values listed at the top of bars represent statistical significance for comparison between 1,25D-treatment and ethanol vehicle control group in each and all cases. Statistical analyses were performed using ANOVA with a post hoc adjustment of P values via the Dunnett test that compares every mean to a control mean and takes into account the scatter of all the groups. a Expression of TPH1 in serotonergic rat raphe RN46A-B14 cells treated with 1,25D. The exact fold-effect values for 1 and 100 nM 1,25D are too small to be perceived in the figure as scaled; they are 0.41 ± 0.22 and 0.73 ± 0.17, respectively. Each mRNA value is the average of nine biological replicates ± std. dev. b Expression of TPH2 in serotonergic rat raphe RN46A-B14 cells treated with 1,25D. The exact fold-effect values for 1 and 100 nM 1,25D are too small to be perceived in the figure as scaled; they are 0.36 ± 0.23 and 0.65 ± 0.31, respectively. Each mRNA value is the average of 30 biological replicates ± std. dev. c Expression of Cyp24a1 in serotonergic rat raphe RN46A-B14 cells treated with 1,25D. Each mRNA value is the average of 20 biological replicates ± std. dev