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Table 4 Validation scoring scheme for candidate tuber intake biomarkers

From: Biomarkers of tuber intake

Food item

Metabolites

Biofluid locations

Questionsa

1

2

3a

3b

4

5

6

7

8

Potato

Solanidine

Plasma

Y

U

U

U

U

U

Y

U

U

Serum

Y

U

Y

Y

U

U

Y

Y

U

Saliva

Y

U

U

U

U

U

Y

Y

U

α-Solanine

Serum

Y

Y

Y

U

U

U

Y

Y

U

α-Chaconine

Serum

Y

Y

Y

U

U

U

Y

Y

U

Total potato alkaloids (α-solanine, α-chaconine and aglycone solanidine)

Serum

Y

U

U

U

U

U

Y

Y

U

Saliva

Y

U

U

U

U

U

Y

Y

U

Sweet potato

Pn 3-Caf·sop-5-glc

Plasma

Y

U

U

U

U

U

Y

Y

U

Urine

Y

N

Y

U

U

U

Y

Y

U

Cy 3-Caf·sop-5-glc

Plasma

Y

U

U

U

U

U

Y

Y

U

Urine

Y

U

Y

U

U

U

Y

Y

U

Pn 3-Caf·sop-5-glc and Cy 3-Caf·sop-5-glc

Plasma

Y

U

Y

U

U

U

Y

Y

U

Cassava

Linamarin

Urine

Y

U

Y

U

U

U

Y

Y

Y

  1. aThe following nine validation criteria questions for biomarkers of food intake were used [8]: Q1: Is the marker compound plausible as a specific BFI for the food or food group (chemical/biological plausibility)? Q2: Is there a dose-response relationship at relevant intake levels of the targeted food (quantitative aspect)? Q3: Is the biomarker kinetics described adequately to make a wise choice of sample type, frequency, and time window (time-response)? a: The single-meal time-response relationship of the BFI has been described for a defined sample type and time window in a meal study. b: (The kinetics of the BFI after repeated intakes has been described for a defined sample type in a meal study) OR (accumulation of the BFI in certain sample types has been observed). Q4: Has the marker been shown to be robust after intake of complex meals reflecting dietary habits of the targeted population (robustness)? Q5: Has the marker been shown to compare well with other markers or questionnaire data for the same food/food group (reliability)? Q6: Is the marker chemically and biologically stable during biospecimen collection and storage, making measurements reliable and feasible? Q7: Are analytical variability (CV %), accuracy, sensitivity, and specificity known as adequate for at least one reported analytical method? Q8: Has the analysis been successfully reproduced in another laboratory (reproducibility)?