Fig. 2
From: Estrogen biosynthesis in cultured skeletal muscle cells (L6) induced by amino acids
a, b Effect of amino acids on estrogenic steroids and MVD protein levels. Quantity of estrogenic steroids (a) and MVD-protein levels (b) increased in L6 cells when cultured in the presence of high AA concentrations (9 mM) compared to low AA concentrations (0.3 mM) in the culture media. Estrone + 18 ± 3%, estradiol + 31 ± 4%, n = 10/group in a, and n = 17/group in b * p < 0.001 vs low AA. Steroid concentrations were determined by GC-MS/MS and MVD proteins by Western blot as described in “Methods” section. Cell numbers following low and high amino acid exposures were equal as confirmed by crystal violet staining (≈ 2 × 107 cells in a)