Transcriptomic changes in peripheral blood mononuclear cells with weight loss: systematic literature review and primary data synthesis

Day, Kaitlin; Dordevic, Aimee L.; Truby, Helen; Southey, Melissa C.; Coort, Susan; Murgia, Chiara

doi:10.1186/s12263-021-00692-6

Table 2 Study characteristics of included studies

From: Transcriptomic changes in peripheral blood mononuclear cells with weight loss: systematic literature review and primary data synthesis

Study	Study design	Sample size	Population^a	Intervention duration	Intervention description	RNA extraction	Microarray	Weight change^a	Transcript response^{b, c}		Pathway response^{b, d}
Study	Study design	Sample size	Population^a	Intervention duration	Intervention description	RNA extraction	Microarray	Weight change^a	Effect of intervention	HR vs LR	Effect of intervention	HR vs LR
Crujeiras et al. 2008 [21]	Cohort	9	44.8 ± 5.2 year, BMI: 44.8 (5.2) kg/m², 100% male, 100% Caucasian	8 weeks	35% energy restriction, 55% carbohydrates, 30% lipids, 15% protein	Trizol	Agilent Human 1A Oligo V2	− 8.9 ± 1.5 %	158 increased 227 decreased^e	NA	NA	7^e
Harvie et al. 2016 [22]	Cohort	20 (HR = 7)	40.3 ± 3.2 years, BMI: 28.3 (3.1) kg/m², 100% female, 96% Caucasian	4–5 weeks (1 full menstrual cycle)	Overall 25% energy restriction with 2 consecutive days 65% energy restriction, 5 days no restriction, restriction days—100 g carbohydrates and 50 g protein, non-restricted days—45% low-GI carbohydrates, 30% lipids, 25% protein	RNeasy plus kit	Affymetrix U133 plus2	− 3.3 kg	0	0	8	20
Pinhel et al. 2017 [23] and Pinhel et al. 2018 [27]	Cohort	13	39 ± 9 years, BMI: 42.5 (7.0) kg/m², 100% female, ethnicity NA	6 months	Roux-en-Y gastric bypass surgery	Phenol/chlorform	Illumina HumanHT-12 V4	− 28.8 kg	7 increased 21 decreased	NA	13	NA
Rendo-Urteaga et al. 2015 [24]	Cohort	12 (HR = 6)	13.17 ± 0.98^f years, BMI-SDS: HR: 3.10 (0.54), LR 4.08 (0.43)^f, 100% male, ethnicity NA	10 weeks	Between 1300 and 9204 kcal/day calculated based on energy requirements and severity of obesity	Trizol	Affymetrix Human 1.1 ST	HR: − 4.47 kg (0.23)^f LR: + 0.75 kg	372 increased 456 decreased	0 increased in HR 23 decreased in HR	53	21
Samblas et al. 2018 [25]	Randomised control trial^g	Discovery = 24, validation = 37	48.4 ± 3.5 years, BMI: discovery: HR: 34.9 (3.0) kg/m², LR: 37.0 (2.1) kg/m², validation: HR: 33.8 (3.6) kg/m² LR: 36.1 (5.0) kg/m², 54% male, ethnicity NA	6 months	30% energy restriction, 40% carbohydrates, 30% lipids, 30% protein	Trizol	Illumina HumanHT-12 V4	Discovery: HR: − 10.5% ± 1.8%, LR: − 5.9% ± 1.7% Validation: HR: − 9.5% ± 2.0%, LR: − 5.7% ± 2.1%	NA	20 increased in HR 136 decreased in HR^e	NA	NA
van Bussel et al. 2019 [26]	Randomised Control Trial	40	60 (50–65)^h years, BMI: 29.2 (3.0) kg/m², 52% male, 100% Caucasian	12 weeks	20% energy restriction, 50% carbohydrates, 35% lipids, 15% protein	Trizol	Affymetrix Human 1.1 ST	− 5.6 ± 2.9 kg	0	NA	15	NA

^aResults are presented as mean (SD) unless otherwise stated
^bresults from quantitative analysis conducted as part of this review unless otherwise stated
^ctranscriptomic response describes transcripts that were significantly differentially expressed defined as an absolute fold change > 1.2 or < − 1.2 and adjusted p value < 0.05
^dpathway response describes wikipathways that were overrepresented defined as pathways with a z-score < 1.96 and 5 or more genes in the pathway with an unadjusted p value < 0.05
^edata reported in article
^fdata presented as mean (standard error of the mean)
^gresults of intervention and control pooled for transcriptomic analysis
^h results presented as mean (range). HR high responders defined as > 5% body weight lost over the intervention period, LR low responders, NA not available, BMI-SDS BMI standard deviation score

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ISSN: 1865-3499

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