Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

Table 3 BestKeeper descriptive statistics and ranking of reference genes in iron-replete and iron-deficient animals in all tissues

	*Hprt*	*Rpl19*	*Rps29*	*Tbp*	*Rpl27*	*Rplp0*	*Ppia*	*Rpl22*	*Actb*	*Gapdh*
Geo mean [CP]	22.62	17.58	17.69	24.24	18.94	19.55	17.76	18.15	16.89	22.60
CV	0.03	0.06	0.06	0.04	0.06	0.06	0.06	0.06	0.07	0.06
Min [CP]	21.13	15.45	15.55	21.67	16.62	17.26	15.48	15.68	14.49	19.18
Max [CP]	24.66	20.73	20.38	26.45	22.36	25.65	20.65	21.15	19.90	25.85
Std dev [± CP]	0.58	0.99	1.00	1.01	1.06	1.10	1.10	1.11	1.18	1.43
Min [x-fold]	− 2.81	− 4.39	− 4.39	− 5.94	− 5.00	− 4.88	− 4.86	− 5.52	− 5.26	− 10.66
Max [x-fold]	4.10	8.85	6.47	4.62	10.67	68.84	7.38	8.00	8.10	9.52
Std dev [± x-fold]	1.49	1.99	2.00	2.01	2.08	2.14	2.15	2.16	2.27	2.70
Ranking	1	2	3	4	5	6	7	8	9	10

Geometric mean (CP), coefficient of variance (CV), and standard deviation (± CP) of the Cq values for putative reference genes. RG are ordered from left to right according to their SD_{Cq value}. Reference genes with a SD_Cq value < 1.0 are considered to be an appropriate reference gene when assessing gene expression in pair-fed and iron-deficient animals. To determine the under- and over-expression of a reference gene relative to the gene’s geometric mean (x-fold), the min, max, and standard deviation are calculated by the BestKeeper software

ISSN: 1865-3499