Fig. 6

Human blood samples were analyzed for mRNA expression using a Nanostring nCounter array (Seattle, WA, USA). A demonstrates the relative gene expression for samples collected in this study using standard violin plots. B demonstrates an overall heat map response of all outcome variables in the study. An associated legend has been included to the right of Fig. 6B to demonstrate what the colors above the heat map represent. This included participant ID, Nanostring cartridge ID, condition, and time point. C demonstrates the relative expression of positive (N = 6) and negative (N = 8) controls across a range of samples analyzed in the study. This quality control analysis demonstrates that the expected values (positive controls) were linear and expressed at different levels across all samples. Positive controls were selected based on different levels of expression. In contrast, none of the samples was determined to have high levels of the negative controls. The conclusion is that analysis of the samples in the present study was within manufacture recommended parameters. D, E, and F demonstrate a volcano plot of genes with significant differential expression at 30, 60, and 90 days, respectively