Fig. 1
From: Paternal high-fat diet altered SETD2 gene methylation in sperm of F0 and F1 mice
Effect of HFD on SETD2 expression in testis and sperm of F0 mice. A Significant weight gain of F0 male mice fed HFD over two months. B Morphological analysis of F0 mice testis in CD group (n = 10) and HFD group (n = 10) with H&E staining. C Detection of SETD2 in F0 mice testis from the two groups (n = 10 in each group) by immunohistochemistry, and D the relative level of SETD2 expression in F0 mouse testis in the two group. E Relative expression of SETD2 mRNA in F0 mouse sperm determined by qPCR (n = 10 in each group). F Determination of SETD2 protein from F0 mouse sperm by western blotting (n = 3 in each group), and G relative expression level of SETD2 protein in F0 mouse sperm from the two group (n = 10 in each group). ** above the bars indicates P < 0.01, and * above the bars indicates P < 0.05