Fig. 2
From: Paternal high-fat diet altered SETD2 gene methylation in sperm of F0 and F1 mice
Paternal HFD altered methylation profile of SETD2 promoter region in sperm from F0 mice. A Genomic structure and relative position of the region sequenced are shown, and a total of 26 sites with at least three CpG sequences in the SETD2 promoter region were selected for methylation profiling. The numbering of the CpGs below the sequence corresponds to each of the CpGs analyzed. The methylation level of the 26 sites was measured by pyrosequencing followed by principal component analysis (B) and heatmap analysis (C). The methylation levels of the two groups are shown for Seq1 (D), Seq2 (E) and Seq3 (F). *** above the bars indicates P < 0.001, ** above the bars indicates P < 0.01, and * above the bars indicates P < 0.05