Fig. 3
From: Paternal high-fat diet altered SETD2 gene methylation in sperm of F0 and F1 mice
Paternal HFD reduced total cell numbers in blastocysts. Representative images of blastocysts in each group showing TUNEL assay results for apoptotic cells (green). Apoptotic index (B), and total cell numbers (C) in blastocysts fertilized by F0 mouse sperm between the two groups. D Correlations between the methylation level of SETD2, apoptotic index, and total cell numbers in blastocysts. Spearman’s correlation coefficients are represented by colors ranging from blue (−1) to red (+1). Pregnancy rate (E), litter size (F), and number of pups born (G) between the two groups is shown. Body weights of the F1 males (H) and females (I) from the two groups. *** above the bars indicates P < 0.001, ** above the bars indicates P < 0.01, and * above the bars indicates P < 0.05