Table 1 Primers used for gene expression assay by RT-qPCR
From: Dysfunction of DMT1 and miR-135b in the gut-testis axis in high-fat diet male mice
Primer name | Primer sequence |
|---|---|
GAPDH F | AGGTCGGTGTGAACGGATTTG |
GAPDH R | TGTAGACCATGTAGTTGAGGTCA |
DMT1 F | TCTATGTGGTGGCTGCAGTG |
DMT1 R | GCTGGTATCTTCGCTCAGCA |
FPN F | GGCACTTTGCAGTGTCTGTG |
FPN R | GTCACCAATGATGGCTCCCA |
Stem-loop F | ATCCAGTGCGTGTCGTG |
miR-135b RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCACATA |
miR-135b R | TGCTTATGGCTTTTCATTCC |
U6 RT | GTCGTATCGACTGCAGGGTCCGAGGTATTCGCAGTCGATACGACAAAAATAT |
U6 F | AGCACATATACTAAAATTGGAACGAT |
U6 R | ACTGCAGGGTCCGAGGTATT |